“Natural Tooth Health is the only company that has scientific proof to show our toothpaste and tooth powder can prevent cavities and remineralize enamel and do it as effectively as fluoride products.”
Here at Natural Tooth Health it has always been our goal to create natural oral care products that prevent cavities and remineralize tooth enamel without the use of harmful chemicals including fluoride.
It’s one thing to have this as our goal and to hear our customers glowing testimonials, but it’s quite another to have this goal backed by scientific research.
In 2021, the University of Texas Health in San Antonio conducted two research studies to look at Natural Tooth Health’s toothpaste and tooth powder’s effectiveness at preventing cavities and remineralizing tooth enamel.
We can now show you scientific proof that our oral care products can prevent cavities and remineralize initial cavities as well as or better than fluoride toothpastes and toothpastes based on synthetic nanosized hydroxyapatite.
We are so excited to share the results with you!
The results are as follows:
1. Caries Prevention
Demineralization (enamel dissolution) occurs when enamel is attacked by acid that comes as by-product of bacteria metabolism of sugar.
The % demineralization shown in the study graph below is how much demineralization that occurred during the study with the use of each product, which is an indication of the severity of the initial caries (early-stage cavity) that developed; the lowest % is best.
Fluoride was overwhelmed by the high amount of acid produced by bacteria, hence its poor protection against cavity under high caries risk condition (frequent sugar intake).
However, the high acidity dissociates the HAP toothpaste and tooth powder to release high amount of calcium and phosphate ions that saturates the tooth surface, thereby inhibiting dissolution (demineralization) of enamel.
The Natural Tooth Health (NTH) toothpaste and tooth powder are more effective than standard fluoride toothpaste in preventing caries (cavities).
This study was done under a high caries risk situation (The bacteria were fed frequently with sugar). This situation exaggerates what normally occurs in the mouth during regular eating.
The Natural Tooth Health products and toothpastes based synthetic nanosized HAP were equally effective in preventing cavities.
2. Remineralization Effect
Remineralization is the increase mineral content in a demineralized enamel surface (measured as increase in surface hardness) due to mineral provided by the reaction of toothpaste ingredients with enamel.
The % Remineralization shown in the graph below shows how much remineralization that occurred during the test; the highest % is optimum.
Natural Tooth Health toothpaste and tooth powder exhibited equal effectiveness (no statistical difference) to fluoride toothpaste and toothpastes based on synthetic nanosized HAP in remineralizing initial caries (early-stage cavities).
Artificial saliva showed significantly lower remineralization.
What Do These Dental Studies Show?
Natural Tooth Health is the only company that has scientific proof to show our tooth powder and toothpaste can prevent cavities and remineralize enamel, and do it as effectively as fluoride products.
We are so excited to share this data with our valued customers.
We hope you feel even better about using them to maximize your oral care with these results.
Keep up your cavity prevention with all our oral care products. Shop today!
Research Study Abstracts:
Comparative study of the caries remineralizing effect of hydroxyapatite-containing toothpaste formulations: a pH cycling study
Minh Tuan Do1, Kan Wang1, Sahar Mohseni1, Andrew Dillow1, Yuko Kataoka1, Nahid Iftikhar1, Netheli Kuruwita2, Bennett T. Amaechi2
1Department of comprehensive dentistry, University of Texas Health Science Center at San Antonio, Texas, USA.
2University of Texas at San Antonio, Texas, USA
Objective: Application of hydroxyapatite as an active ingredient in oral care products is increasingly gaining attention. This in vitro study compared the efficacy of natural HAP (natHAP), synthetic HAP and fluoride in remineralizing initial caries. Materials and methods: Initial caries lesions were produced on 105 bovine enamel blocks by 5-day demineralization in a Microbial Caries Model using Todd Hewitt’s broth growth media inoculated with Streptococcus mutans and Lactobacilli acidophilus and fed 3 times daily with 10% sucrose treatment (6 min/episode). The 105 caries-bearing blocks were stratified among 7 treatment groups (15/group); natHAP tooth powder, natHAP-low toothpaste, natHAP-high toothpaste, natHAP-UG toothpaste, synthetic nanosize HAP (nanoHAP), Fluoride toothpaste (NaF), and Artificial saliva (AS). The groups were subjected to remineralization for 28 days using a pH-cycling model with daily regimen of three 2-minute toothpaste slurry treatments, one 5-min acidic challenge, and artificial saliva storage for the rest of the day. Surface microhardness (SMH) of each block was measured after demineralization (SMH,) and after remineralization (SMHR). Paired t-test was used for intra-group comparison (SMH, vs SMHR) while Tukey’s multiple comparison tests were used for inter-group comparisons of the mean percentage remineralization (%Rem). Results: Paired t-test indicated statistically significant (p<0.001) remineralization in all groups. Inter-group comparison showed no statistically significant difference among the groups in their achieved %Rem, except that the %Rem achieved with artificial saliva (9.1±6.28%) was significantly lower than those of natHAP-high (30.61±21.92%) and Fluoride (28.43±15.78%) toothpastes. No statistically significant differences in %Rem among the natHAP formulations or between the natural and synthetic HAP formulations. The natHAP formulations exhibited dose-dependent efficacy, with natHAP-high inducing the highest %Rem. Conclusions: Within the limit of this study, toothpaste based on either natural or synthetic HAP have comparable efficacy to standard fluoride toothpaste in remineralizing initial caries. Natural and synthetic hydroxyapatite were equally effective in remineralizing initial caries.
Caries prevention by toothpaste containing natural hydroxyapatite: a microbial study
Kan Wang’, Minh Tuan Do’, Sahar Mohseni’, Andrew Dillow’, Yuko Kataoka’, Nahid Iftikhar1, Netheli Kuruwita2, Bennett T. Amaechi2
‘Department of comprehensive dentistry, University of Texas Health Science Center at San Antonio, Texas, USA.
2University of Texas at San Antonio, Texas, USA
Objective: Use of non-fluoride toothpaste is gaining much attention, and hydroxyapatite (HAP) is one of the most favored alternatives to fluoride. This in vitro study investigated whether toothpaste based on HAP is effective in preventing dental caries by comparing the efficacy, in preventing tooth surface demineralization, of toothpaste formulations containing various forms of HAP. Methods: 140 sound bovine enamel blocks were assigned to 7 treatment groups (20/group); natHAP tooth powder, natHAP-low toothpaste, natHAP-high toothpaste, natHAP- UG toothpaste, synthetic nanosize HAP (nanoHAP), Fluoride toothpaste (NaF), and untreated control (control). All blocks were treated with whole human saliva for 60 minutes to develop acquired salivary pellicle, after which the blocks were subjected to 5-day demineralization for initial caries development in a Microbial Caries Model (MCM) using Todd Hewitt’s broth growth media (THB) inoculated with Streptococcus mutans and Lactobacilli acidophilus with daily regimen of two 2-minute toothpaste slurry treatments, three 6-minute 10% sucrose treatment, and in THB for the rest of the day. Surface microhardness (SMH) of each block was measured before and after treatment as a measure of the amount of demineralization. Data were analyzed by paired t-test for intra-group comparison and Tukey’s HSD for inter-group multiple comparison. Results: There was a significant (p<0.05) demineralization in all groups, however, percentage demineralization (%Dem) was significantly lower (p<0.001) in natHAP formulations than in untreated control. Standard 1100 ppm fluoride toothpaste (62.54±4.88%) did not differ significantly from the control (69.81±5.82%). There were no statistically significant differences in %Dem among the natHAP formulations or between the natural and synthetic HAP formulations. Conclusions: Toothpastes based on HAP are more efficacious than standard fluoride toothpaste in preventing caries in high caries risk situation. Natural and synthetic hydroxyapatite are equally effective in preventing caries.